Detection of antifungal compounds in Arabidopsis thaliana and brassica oleracea by thin layer chromatography .
PDF

Keywords

Thin Layer Chromatography
phytoalexin
camalexin
brassilexin
Pseudomonas syringae pv. maculicola

Abstract

Thin Layer Chromatography (TLC) was applied to detect antifungal compounds both in Arabidopsis thaliana and Brassica oleracea after elicited by various biotic and abiotic elicitors.From TLC bioassays the only strong zone of inhibition detected after challenged by Pseudomonas syringae pv.maculicola was from Arabidopsis tissue and later confirmed by spectrophotometry as camalexin but no corresponding phytoalexin was found in broccoli leaf.
https://doi.org/10.29037/ajstd.99
PDF

References

Bennett, R.N. and Wallsgrove, R.M. (1994), Secondary metabolites in plant defence mechanisms, New Phytologist, vol. 127, pp. 617-633.

Chong, K.P. (2005), Role of phytoalexin in Arabidopsis thaliana and Brassica oleracea against Botrytis cinerea, MRes thesis. Imperial College, University of London.

Denby, K.J., Kumar, P. and Kliebenstein, D.J. (2004), Identification of Botrytis cinereasusceptibility loci in Arabidopsis thaliana, The Plant Journal, vol. 38, pp. 473-486.

Dixon, R.A. and Harrison, M.J. (1990), Activation, structure and organization of genes involved in microbial defense in plants, Advance Genetics, vol. 28, pp. 165-234.

Ferrari, S., Plotnikova, J.M., De Lorenzo, G., and Ausubel, F.M. (2003),Arabidopsis local resistance to Botrytis cinerea involves salicylic acid and camalexin requires EDS4 and PAD2, but not SID2, EDS5 or PAD4, Plant Journal, vol. 35, pp. 193-205.

Govrin, E.M. and Levine, A. (2002), Infection of Arabidopsis

with a necrotrophic pathogen Botrytis cinerea, elicits various defense responses but does not induce systemic acquires resistance (SAR), Plant Molecular Biology, vol. 48, pp. 267-276.

Grayer, R.J. and Harborne, J.B. (1994), A survey of antifungal compounds from plants,1982-1993, Phytochemistry, vol. 37, pp. 19-42.

Kagan, I. and Hammerschmidt, R. (2002), Arabidopsis ecotype variability in camalexin production and reaction to infection by

Alternaria brassicicola,Journal of Chemistry Ecology, vol. 28, pp.2121-2140.

Kliebenstein, D.J. (2004), Secondary metabolites and plant/environment interactions: A view through Arabidopsis thaliana tinged glasses, Plant, Cell and Environment, vol. 27,

pp. 675-684.

Mansfield, J.W. (2000), Antimi crobial compounds and resistance.

In: Slusarenko, A. Fraser, R.S.S. and Van Loon, L.C. (Eds.) (2000), Mechanism of Resistance to Plant Diseases, Netherlands, Kluwer Academic Publishers.

Miedzybrodzki, R. (2003), Biological activity of the isothiazole derivatives, Postepy Hig Med Dosw, vol. 57, pp. 617-630.

Maor, R. and Shirasu, K. (2005), The arms race continues battle strategies between plants and fungal pathogens, Current Opinion in Microbiology, vol. 8, pp. 399-404.

Paxton, J. (1981), Phytoalexins - a working redefinition, Journal of phytopathology, vol. 101, pp. 106-109.

Pedras, M.S.C., Montaut, S., and Suchy, M. (2004), Phytoalexins from the Crucifer Rutabaga: Structures, Syntheses, Biosyntheses, and Antifungal Activity, Journal of Organic Chemistry, vol. 69, pp.4471-4476.

Reuber, T.L., Plotnikova, J.M., Dewdney, J., Rogers, E.E., Wood, W., and Ausubel, F.M. (1998), Correlation of defense gene induct

ion with powdery mildew susceptibility in Arabidopsis enhanced disease susceptibility mutants, Plant Journal, vol. 16, pp. 473-487.

Rhodes, D. (2003), Secondary products derived from aromatic Amino acids [ONLINE] Available from: http://www.hort.purdue.edu/rhodcv/hort640c/secprod/se00001.html [Accessed 26June 2005].

Roetschi, A., Si-Ammour, A., Belbahri, L., Mauch, F., and Mauch-Mani, B. (2001), Characterization of an Arabidopsis-phytopthora pathosystem: Resistance requires a functional PAD2 gene and is independent of salicyclic acid, ethylene and Jasmonic acid

signaling, Plant Journal, vol. 28, pp. 293-305.

Rouxel, T., Kollmann, A., Boulidard, L., and Mithen, R. (1991), Abiotic elicitation of indole phytoalexins and resistance to maculans within Brassiceae, Planta, vol. 184, pp. 271-278.

Soylu, S. (1998), Analysis of the responses of Arabidopsis thaliana to infection by Albugo candida and pathovars of Pseudomonas syringae, PhD thesis. Wye College, University of London.

Thomma, B.P., Nelissen, I., Eggermont, K., nd Broekaert, W.F. (1999), Deficiency in phytoalexin production causes enhanced susceptibility of Arabidopsis thaliana to the fungus Alternaria brassicicola, Plant Journal, vol. 19, pp. 163-171.

Tsuji, J., Jackson, E.P., Gage, D.A., Hammerschmidt, R., and Somerville, S.C. (1992), Phytoalexin accumulation in Arabidopsis thaliana during the hypersensitive reaction to Pseudomonas syringae pv. syringae, Plant Physiology, vol. 98, pp.1304-1309.

Zook, M., Leege, L., Jacobson, D., and Hamerschmidt, R. (1998), Camalexin accumulation in Arabis lyrata,Phytochemistry, vol. 49, pp. 2287-2289.

Downloads

Download data is not yet available.