The ferrite (a Fe3O4 – gFe2O3 mixed solution) fine particles, ~8 nm in size were synthesized from an aqueous solution. Trypsin, a proteolytic enzyme or a protein, was immobilized onto the surfaces of those particles during the synthesis process. The process was performed in the open air at a temperature as low as 4°C and on near-neutral condition of pH = 9, which is compatible with most of the bioactive molecules as well as trypsin. Therefore this technique is advantageous for preparing magnetite particles having biomolecules immobilized on their surfaces, which will be used for biomedical applications utilizing magnetic separation technique.
Tran Hoang Hai (2005), Magnetic nanoparticles and their applications in biomedicine, The second German-Vietnam Summer Schoole on Modern Optics and New Materials, Ho Chi Minh City, 151.
Cohen, S.A. and Strydom, D.J. (1988), Anal. Biochem. 174, 1.3. Lottenberg, R., Christensen, U., Jackson, C.M., and Coleman, P.L. (1981), Methods Enzymol. 80, 341.