MEDIUM OPTIMIZATION FOR THE PRODUCTION OF BIOMASS BY Cunninghamella sp. 2A1 USING RESPONSE SURFACE METHODOLOGY
PDF

Keywords

Medium optimization
Cunninghamella sp. 2A1
Biomass
Response surface.

Abstract

A statistical design approach has been used to optimize the production of biomass byCunninghamellasp. 2A1, evaluated based on lipidless biomass. A 23full factorial centralcomposite design (CCD) was chosen to study the combined effects of three factors; ammoniumtartrate, peptone and glucose concentrations. The p-value for each factors was < 0.05 suggestingthat these factors have significant effect on the production of lipidless biomass. The productionis represented by a linear model with p-value < 0.0001. The optimized medium consists of 3.86g/l ammonium tartrate, 55.84 g/l glucose and 7.73 g/l peptone predicted 16.83 g/l lipidlessbiomass. Results from four replications based on the optimized medium produced 18.48 g/llipidless biomass, which are in close agreementwith the predicted value. The coefficient forglucose was the highest indicating it as the most significant factoraffecting lipidless biomassproduction.

 

https://doi.org/10.29037/ajstd.160
PDF

References

Ratledge, C. (1992), Microbial lipids: commercial realities or academic curiosities, In: Kyle, D.J. and Ratledge, C. (Eds.), Industrial applications of single cell oils, AOCS Press, Champaign, pp. 1-14.

Maddon, I.S. and Richard, S.H. (1977), Use of response surface methodology for rapid optimization of microbiological media, Journal of Applied Bacteriology, vol. 43, pp. 197-204.

Lee, S.L. and Chen, W.C. (1997), Optimization of medium composition for the production of glucosyltransferase by Aspergillus niger with response surface methodology, Enzyme and Microbial Technology, vol. 21, pp. 436-440.

Rahman, R.A., Illias, R.M., Nawawi, M.G.M., Ismail, A.F., Hassan, O., and Kamaruddin, K. (2004), Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology, Process Biochemistry, vol. 39, pp. 2053-2060.

Ibrahim, H.M., Yusoff, W.M.W., Hamid, A.A., Illias, R.M., Hassan, O., and Omar, O. (2005), Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD), Process Biochemistry, vol. 40, pp. 753 758.

Chang, Y.N., Huang, J.C., Lee, C.C., Shih, I.L., and Tzeng, Y.M. (2002), Use of response surface methodology to optimize culture medium for the production of lovastatin by Monascus rubber,Enzyme and Microbial Technology, vol. 30, pp. 889-894.

Kendrick, A. and Ratledge, C. (1992), Desaturation of polyunsaturated fatty acids in Mucor circinelloides and the involvement of the novel membrane-bound malic enzyme, European Journal of Biochemistry, vol. 209,pp. 667-673.

Chaney, A.L. and Marbach, E.P. (1962), Modified reagents for determination of urea and ammonium, Clinical Chemistry, vol. 8,pp. 130-132.

Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957), A simple method for the isolation of total lipids from animal tissues, Journal of Biological Chemistry, vol. 226,pp. 497-506.

Myers, R.H. and Montgomery, D.C. (1995), Response surface methodology: process and product optimization using designed experiments. Wiley-Interscience.

Haalland, P.D. (1989), Experimental design in biotechnology, New York, Marcel Dekker, Inc.

Khuri, A.I. and Cornell, J.A. (1987), Response surfaces: design and analysis, New York, Marcel Dekker, Inc.

Stanbury, P.F., Whitaker, A., and Hall, S.J. (1984), Principles of fermentation technology, 2 (Ed.) Butterworth-Heinemann, Oxford.

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2017 Siti Aminah Sulaiman, Aidil Abdul Hamid, Wan Mohtar Wan Yusoff

Downloads

Download data is not yet available.