Eight isolates of entomopathogenic fungi were evaluated as dried conidia against the rice moth, Corcyra cephalonica. In bioassays two isolates of Beauveria bassiana (BbGc and BbPs) and one isolate of Metarhizium anisopliae (MaPs) consistently gave high mortality to C. cephalonica larvae. Formulations in either kaolin, talc or tapioca flour (20 % w/w a.i.) thoroughly mixed with long grain rice in plastic cups (8 cm diameter by 5 cm) gave complete larval mortality by the 12th day of treatment. However, in general those formulated in kaolin and talc were more efficacious and faster to kill compared to those formulated in tapioca flour or the unformulated control. Even at the lowest rate of 0.05 g BbGc in kaolin provided 100% mortality 7 days after introduction compared with other dust formulations. Isolate BbGc in kaolin and talc administered at 0.4 g a.i. in 200 g rice packed in plastic kept at room temperature provided protection against the rice moth up to 4 months of storage. Larval mortality in excess of 90% was obtained 15 days after introduction. Formulations of MaPs was effective only within the first month of storage beyond which infectivity rapidly declined.
Adane, K., Moore, D., and Archer, S.A. (1996), Preliminary studies on the use of Beauveria bassiana to control Sitophilus zeamais (Coleoptera: Curculionidae) in the Laboratory. Journal of Stored Product Reasearch, vol. 32, pp. 105-113.
Belloa, G.D., Padina, S., Lastrab, C.L., and Fabrizio, M. (2000), Laboratory evaluation of chemical biological control of rice weevil (Sitophilus oryzae L.) in store grain. Journal of Stored Product Reasearch, vol. 37, pp. 77-84.
Daoust, R.A., Ward, M.G., and Robert, D.W. (1983), Effect of formulation on the viability of Metarhizium anisopliae conidia. Journal of Invertebrate Pathology, vol. 41, pp.151-160.
Ekesi, S., Egwurube, E.A., Akpa, A.D., and Onu, I. (2001), Laboratory evaluation of the entomopathogenic fungus, Metarhizium anisopliae for the control of the groundnut bruchid, Caryedon serratus on groundnut. Journal of Stored Product Research, vol. 37, pp. 313-321.
EPA (2001), Protection of stratospheric ozone: process for exempting quarantine and preshipment application of methyl bromide. United States Environmental Protection Agency, Federal Register, vol. 66, pp. 37752-37769.
Finney, D.J. (1971), Probit analysis. Cambridge Univ. Press, Cambridge, UK.
Gomez, K.A. and Gomez, A.A. (1984), Statistical Procedures for Agricultural Research. John Wiley and Sons, Inc.
Hallsworth, J.E. and Magan, N. (1999), Water and Temperature Relations of Growth of the Entomogenous Fungi Beauveria bassiana, Metarhizium anisopliae and Paecilomyces farinosus. Journal of Invertebrate Pathology, vol. 74, pp. 261-266.
Hidalgo, E., Moore, D., and Patourel, G. LE. (1998), The effect of different formulation of Beauveria bassiana on Sitophilus zeamais in store maize. Journal of Stored Product Research, vol. 34, pp. 171-179.
Hodges, R.J. (1979), A review of the biological and control of the rice moth Corcyra cephalonica Stainton (Lepidoptera: Gallerinae). Tropical Product Institute 56/26 Gray`s Inn Road, London.
Khan, A.R. and Selman, B.J. (1989), Nosema spp. (Microspora: Microsporida: Noesematidae) of stored product Coleoptera and their potential as microbial control agents. Agricultural Zoology Reviews, vol. 3, pp. 193-223.
Moy, J.H. (1988), Irradiation as a substitute to chemical fumigation of food. IAEATECDOC vol. 452, pp. 17-33.
Osman, N. and Nordin, N. (1990), Diet untuk pemeliharaan kupu-kupu beras, Corcyra cephalonica St. (Lepidoptera: Pyralidae). Pertanika, vol. 13, pp. 205-209.
Rice, W.C. (1999), Activity of the entomopathogenic fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) against three coleopteran pest of stored grain. Journal of Economic Entomology, vol. 92, pp. 691-694.
Smith, S.M., Moore, D., Karanja, L., and Chandi, E.A. (1999), Formulation of vegetable fat pellets with pheromone and Beauveria bassiana to control the larger grain borer, Prostephanus truncatus (Horn.). Pesticide Science, vol. 55, pp. 711-718.
Tanya, S. and Doberski, J. (1984) An investigation of the entomogenous fungus Beauveria bassiana (Bals.) Vuill. as a potential biological control agent for Oryzaephilus surinamensis (L.). Journal of Stored Product Reasearch, vol. 20, pp. 17-23.
Ward, M.G. (1984), Formulation of Biological Insecticides. American Chemical Society.